Uterorelaxant Activity of Laurus nobilis L. Leaf Extract on Mouse Uterine Contractility
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Abstract
Background: The leaves of Laurus nobilis are traditionally used in folk medicine for their anti-inflammatory, antinociceptive, muscle relaxant properties and relief of menstrual pain. The effect of L. nobilis on uterine contractility remains poorly understood. This study investigated the relaxant effects of Laurus nobilis leaf extract (LNE) on isolated non-pregnant mouse uterus and explored its possible underlying mechanisms.
Methods: The effects of increasing concentrations of LNE (6.25–400 ?g/mL) on spontaneous contractions and contractions induced by oxytocin, prostaglandin F?? (PGF??), methacholine, high potassium chloride (KCl), and oxytocin in calcium-free medium were evaluated. Additional experiments were performed in the presence of propranolol or tetraethylammonium.
Results: LNE significantly reduced spontaneous uterine contractions in a concentration-dependent manner. The extract significantly suppressed uterine contractions induced by oxytocin, PGF??, methacholine, and high KCl. The inhibitory effect was not attenuated by propranolol or tetraethylammonium.
Conclusion: These findings demonstrate that LNE possesses significant uterorelaxant activity in the isolated nonpregnant mouse uterus, possibly mediated through inhibition of extracellular calcium influx. These findings support the potential pharmacological relevance of L. nobilis in the management of conditions associated with excessive uterine contractility, such as dysmenorrhea.
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